Tomato hybrid SVTM2303

ABSTRACT

The invention provides seed and plants of tomato hybrid SVTM2303. The invention thus relates to the plants, seeds, and tissue cultures of tomato hybrid SVTM2303 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.15/376,441, filed Dec. 12, 2016, now U.S. Pat. No. 10,198,298 whichclaims the priority of U.S. Provisional Appl. Ser. No. 62/416,042, filedNov. 1, 2016, the entire disclosures of which are incorporated herein byreference.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid SVTM2303 and theinbred tomato lines PSQ9Z14-9091 and PSQ9Z12-9063.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of thehybrid designated SVTM2303, the tomato line PSQ9Z14-9091 or tomato linePSQ9Z12-9063. Also provided are tomato plants having all thephysiological and morphological characteristics of such a plant. Partsof these tomato plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid SVTM2303and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063 comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of tomato hybrid SVTM2303 and/ortomato lines PSQ9Z14-9091 and PSQ9Z12-9063 is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of tomato hybrid SVTM2303 and/ortomato lines PSQ9Z14-9091 and PSQ9Z12-9063. The tomato seed of theinvention may be provided as an essentially homogeneous population oftomato seed of tomato hybrid SVTM2303 and/or tomato lines PSQ9Z14-9091and PSQ9Z12-9063. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, insome embodiments, seed of hybrid SVTM2303 and/or tomato linesPSQ9Z14-9091 and PSQ9Z12-9063 may be defined as forming at least about97% of the total seed, including at least about 98%, 99% or more of theseed. The seed population may be separately grown to provide anessentially homogeneous population of tomato plants designated SVTM2303and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid SVTM2303 and/or tomato linesPSQ9Z14-9091 and PSQ9Z12-9063 is provided. The tissue culture willpreferably be capable of regenerating tomato plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant, and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of the hybrid SVTM2303and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063 include those traitsset forth in the tables herein. The regenerable cells in such tissuecultures may be derived, for example, from embryos, meristems,cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers,seed and stalks. Still further, the present invention provides tomatoplants regenerated from a tissue culture of the invention, the plantshaving all the physiological and morphological characteristics of hybridSVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent tomatoplants is a plant of tomato line PSQ9Z14-9091 or tomato linePSQ9Z12-9063. These processes may be further exemplified as processesfor preparing hybrid tomato seed or plants, wherein a first tomato plantis crossed with a second tomato plant of a different, distinct genotypeto provide a hybrid that has, as one of its parents, a plant of tomatoline PSQ9Z14-9091 or tomato line PSQ9Z12-9063. In these processes,crossing will result in the production of seed. The seed productionoccurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid SVTM2303 and/or tomatolines PSQ9Z14-9091 and PSQ9Z12-9063. In one embodiment of the invention,tomato seed and plants produced by the process are first generation (F₁)hybrid tomato seed and plants produced by crossing a plant in accordancewith the invention with another, distinct plant. The present inventionfurther contemplates plant parts of such an F₁ hybrid tomato plant, andmethods of use thereof. Therefore, certain exemplary embodiments of theinvention provide an F₁ hybrid tomato plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SVTM2303 and/or tomato linesPSQ9Z14-9091 and PSQ9Z12-9063, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SVTM2303 and/or tomatolines PSQ9Z14-9091 and PSQ9Z12-9063, wherein said preparing comprisescrossing a plant of the hybrid SVTM2303 and/or tomato lines PSQ9Z14-9091and PSQ9Z12-9063 with a second plant; and (b) crossing the progeny plantwith itself or a second plant to produce a seed of a progeny plant of asubsequent generation. In further embodiments, the method mayadditionally comprise: (c) growing a progeny plant of a subsequentgeneration from said seed of a progeny plant of a subsequent generationand crossing the progeny plant of a subsequent generation with itself ora second plant; and repeating the steps for an additional 3-10generations to produce a plant derived from hybrid SVTM2303 and/ortomato lines PSQ9Z14-9091 and PSQ9Z12-9063. The plant derived fromhybrid SVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063 may bean inbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid SVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063is obtained which possesses some of the desirable traits of theline/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid SVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063,wherein the plant has been cultivated to maturity, and (b) collecting atleast one tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid SVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063is provided. The phrase “genetic complement” is used to refer to theaggregate of nucleotide sequences, the expression of which sequencesdefines the phenotype of, in the present case, a tomato plant, or a cellor tissue of that plant. A genetic complement thus represents thegenetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SVTM2303 and/or tomato lines PSQ9Z14-9091and PSQ9Z12-9063 could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of tomato hybrid SVTM2303, tomato linePSQ9Z14-9091 and tomato line PSQ9Z12-9063.

A. Origin and Breeding History of Tomato Hybrid SVTM2303

The parents of hybrid SVTM2303 are PSQ9Z14-9091 and PSQ9Z12-9063. Theparent lines are uniform and stable, as is a hybrid produced therefrom.A small percentage of variants can occur within commercially acceptablelimits for almost any characteristic during the course of repeatedmultiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Tomato HybridSVTM2303, Tomato Line PSQ9Z14-9091 and Tomato Line PSQ9Z12-9063

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of tomato hybrid SVTM2303 and the parent lines thereof.A description of the physiological and morphological characteristics ofsuch plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSVTM2303 Comparison: Characteristic SVTM2303 HyPeel 303 1. Seedlinganthocyanin in hypocotyl of 2-15 cm present present seedling habit of3-4 week old seedling normal normal 2. Mature Plant height 53.1 cm 53.43cm growth type determinate determinate form normal compact size ofcanopy (compared to others of large medium similar type) habitsemi-erect semi-erect 3. Stem branching profuse intermediate branchingat cotyledon or first leafy present present node number of nodes betweenfirst 4 to 7 4 to 7 inflorescence number of nodes between early (1st to1 to 4 1 to 4 2nd, 2nd to 3rd) inflorescences number of nodes betweenlater 1 to 4 1 to 4 developing inflorescences pubescence on youngerstems moderately hairy sparsely hairy 4. Leaf type (mature leaf beneaththe 3rd tomato tomato inflorescence) margins of major leaflets (matureleaf shallowly toothed or shallowly toothed or beneath the 3rdinflorescence) scalloped scalloped marginal rolling or wiltiness (matureslight moderate leaf beneath the 3rd inflorescence) onset of leafletrolling (mature leaf early season mid season beneath the 3rdinflorescence) surface of major leaflets (mature leaf rugose (bumpy orrugose beneath the 3rd inflorescence) veiny) pubescence (mature leafbeneath the normal normal 3rd inflorescence) 5. Inflorescence type (makeobservation on the 3rd simple forked inflorescence) average number offlowers in 7.3 8.5 inflorescence (3rd inflorescence) leafy or “running”inflorescence (3rd absent absent inflorescence) 6. Flower calyx normal(lobes awl normal shaped) calyx-lobes shorter than corolla shorter thancorolla corolla color yellow yellow style pubescence absent sparseanthers all fused into tube all fused into tube fasciation (1st flowerof 2nd or 3rd absent absent inflorescence) abscission layer absent(jointless) absent 7. Fruit surface smooth smooth base color(mature-green stage) apple or medium apple or medium green green pattern(mature-green stage) uniform green uniform green shape of transversesection (3rd fruit of flattened round 2nd or 3rd cluster) shape ofblossom end (3rd fruit of 2nd nippled flat or 3rd cluster) shape of stemend (3rd fruit of 2nd or flat indented 3rd cluster) shape of pistil scar(3rd fruit of 2nd or dot dot 3rd cluster) point of detachment of fruitat harvest at calyx attachment at calyx attachment (3rd fruit of 2nd or3rd cluster) stem scar size medium medium length of mature fruit (3rdfruit of 2nd 64.49 mm 68.64 mm or 3rd cluster) (length of mature fruit(stem axis)) diameter of mature fruit (3rd fruit of 46.88 mm 50.73 mm2nd or 3rd cluster) (diameter of fruit at widest point) weight of maturefruit (3rd fruit of 2nd 83.7 g 91.8 g or 3rd cluster) core presentpresent number of locules three and four three and four color, full ripered red flesh color, full-ripe red/crimson red/crimson flesh color withlighter and uniform darker areas in wall locular gel color of table-ripefruit yellow red ripening (blossom-to-stem axis) uniformblossom-to-stem-end ripening (peripheral-to-central-radial axis) insideout inside out epidermis color yellow yellow epidermis easy-peeleasy-peel epidermis texture tender tender thickness of pericarp 6.79 mm6.64 mm 8. Phenology seeding to 50% flow (1 open on 50% 63 64 of plants)(days) seeding to once over harvest (days) 114 113 fruiting season veryconcentrated medium 9. Adaptation culture field field 10. Chemistry andComposition of Full-Ripe n/a n/a Fruits pH 4.3 4.3 titratable acidity,as % citric 0.3745 0.398 total solids (dry matter, seeds and skin 6.75.85 removed) (percentage total content) soluble Solids as °Brix 5.855.15 *These are typical values. Values may vary due to environment.Other values that are substantially equivalent are also within the scopeof the invention.

TABLE 2 Physiological and Morphological Characteristics of LinePSQ9Z14-9091 Comparison: Characteristic PSQ9Z14-9091 PSQ-24-988 1.Seedling anthocyanin in hypocotyl of 2-15 cm present present seedlinghabit of 3-4 week old seedling normal normal 2. Mature Plant height56.53 cm 40.8 cm growth type determinate determinate form normal lax,open size of canopy (compared to others of large large similar type)habit semi-erect sprawling 3. Stem branching intermediate profusebranching at cotyledon or first leafy present present node number ofnodes between first 4 to 7 7 to 10 inflorescence number of nodes betweenearly (1st to 1 to 4 1 to 4 2nd, 2nd to 3rd) inflorescences number ofnodes between later 1 to 4 4 to 7 developing inflorescences pubescenceon younger stems densely hairy or wooly moderately hairy 4. Leaf type(mature leaf beneath the 3rd tomato tomato inflorescence) margins ofmajor leaflets (mature leaf shallowly toothed or shallowly toothed orbeneath the 3rd inflorescence) scalloped scalloped marginal rolling orwiltiness (mature strong strong leaf beneath the 3rd inflorescence)onset of leaflet rolling (mature leaf mid season mid season beneath the3rd inflorescence) surface of major leaflets (mature leaf rugose (bumpyor rugose beneath the 3rd inflorescence) veiny) pubescence (mature leafbeneath the normal hirsute 3rd inflorescence) 5. Inflorescence type(make observation on the 3rd simple simple inflorescence) average numberof flowers in 7.68 6.2 inflorescence (3rd inflorescence) leafy or“running” inflorescence (3rd absent absent inflorescence) 6. Flowercalyx normal (lobes awl normal (lobes awl shaped) shaped) calyx-lobesapprox. equaling shorter than corolla corolla corolla color yellowyellow style pubescence sparse absent anthers all fused into tube allfused into tube fasciation (1st flower of 2nd or 3rd absent absentinflorescence) abscission layer absent (jointless) absent (jointless) 7.Fruit surface smooth smooth base color (mature-green stage) apple ormedium apple or medium green green pattern (mature-green stage) uniformgreen uniform green shape of transverse/cross section (3rd roundirregular fruit of 2nd or 3rd cluster) shape of blossom end nipplednippled shape of stem end (3rd fruit of 2nd or flat flat 3rd cluster)shape of pistil scar (3rd fruit of 2nd dot dot or 3rd cluster) point ofdetachment of fruit at harvest at calyx attachment at calyx attachment(3rd fruit of 2nd or 3rd cluster) stem scar size medium medium length ofmature fruit (3rd fruit of 2nd 65.92 mm 64.47 mm or 3rd cluster) (lengthof mature fruit (stem axis)) diameter of mature fruit (3rd fruit of47.02 mm 47.59 mm 2nd or 3rd cluster) (diameter of fruit at widestpoint) weight of mature fruit (3rd fruit of 82.89 g 75.26 g 2nd or 3rdcluster) core present present number of locules three and four three andfour color, full ripe red red flesh color, full-ripe red/crimsonred/crimson flesh color uniform uniform locular gel color of table-ripefruit red yellow ripening (blossom-to-stem axis) uniform uniformripening (peripheral-to-central-radial axis) uniformity uniformityepidermis color yellow yellow epidermis normal easy-peel epidermistexture average tender thickness of pericarp 6.7 mm 6.3 mm 8. Phenologyseeding to 50% flow (1 open on 50% 83 63 of plants) (days) seeding toonce over harvest (days) 114 122 fruiting season long very concentrated9. Adaptation culture field field 10. Chemistry and Composition ofFull-Ripe Fruits pH 4.25 4.1 titratable acidity, as % citric 0.37 0.493total solids (dry matter, seeds and skin 7.3 7.05 removed) (percentagetotal content) soluble Solids as °Brix 6.2 5.9 *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.

TABLE 3 Physiological and Morphological Characteristics of LinePSQ9Z12-9063 Comparison: HP 988 Characteristic PSQ9Z12-9063 (PSQ24-988) 1. Seedling anthocyanin in hypocotyl of 2-15 cm present presentseedling habit of 3-4 week old seedling normal normal 2. Mature Plantheight 47.1 cm 38.9 cm growth type determinate determinate form lax,open lax, open size of canopy (compared to others of medium mediumsimilar type) habit sprawling sprawling 3. Stem branching intermediatesparse branching at cotyledon or first leafy absent absent node numberof nodes between first 1 to 4 4 to 7 inflorescence number of nodesbetween early (1st to 1 to 4 1 to 4 2nd, 2nd to 3rd) inflorescencesnumber of nodes between later 1 to 4 1 to 4 developing inflorescencespubescence on younger stems moderately hairy sparsely hairy 4. Leaf type(mature leaf beneath the 3rd tomato tomato inflorescence) margins ofmajor leaflets (mature leaf shallowly toothed or shallowly toothed orbeneath the 3rd inflorescence) scalloped scalloped marginal rolling orwiltiness (mature moderate moderate leaf beneath the 3rd inflorescence)onset of leaflet rolling (mature leaf mid season mid season beneath the3rd inflorescence) surface of major leaflets (mature leaf rugose (bumpyor rugose beneath the 3rd inflorescence) veiny) pubescence (mature leafbeneath the normal normal 3rd inflorescence) 5. Inflorescence type (makeobservation on the 3rd simple simple inflorescence) average number offlowers in 6.5 6.3 inflorescence (3rd inflorescence) leafy or “running”inflorescence (3rd occasional occasional inflorescence) 6. Flower calyxnormal (lobes awl normal shaped) calyx-lobes shorter than corollaapprox. equaling corolla corolla color yellow yellow style pubescenceabsent absent anthers all fused into tube all fused into tube fasciation(1st flower of 2nd or 3rd absent absent inflorescence) abscission layerabsent (jointless) absent 7. Fruit surface smooth smooth base color(mature-green stage) light green apple or medium green pattern(mature-green stage) uniform green uniform green shape of transversesection (3rd fruit of round flattened 2nd or 3rd cluster) shape ofblossom end (3rd fruit of 2nd tapered tapered or 3rd cluster) shape ofstem end (3rd fruit of 2nd or indented indented 3rd cluster) shape ofpistil scar (3rd fruit of 2nd or dot dot 3rd cluster) point ofdetachment of fruit at harvest at calyx attachment at pedicel joint (3rdfruit of 2nd or 3rd cluster) stem scar size medium large length ofmature fruit (3rd fruit of 2nd 58.1 mm 61.4 mm or 3rd cluster) (lengthof mature fruit (stem axis)) diameter of mature fruit (3rd fruit of 46.8mm 48.9 mm 2nd or 3rd cluster) (diameter of fruit at widest point)weight of mature fruit (3rd fruit of 2nd 61.7 g 83.1 g or 3rd cluster)core present present number of locules two three and four color, fullripe red red flesh color, full-ripe red/crimson red/crimson flesh colorwith lighter and with lighter and darker areas in walls darker areas inwall locular gel color of table-ripe fruit red red ripening(blossom-to-stem axis) uniform uniform ripening(peripheral-to-central-raidal axis) uniformity uniformity epidermiscolor yellow yellow epidermis normal normal epidermis texture averageaverage thickness of pericarp 5.4 mm 6.5 mm 8. Phenology seeding to 50%flow (1 open on 50% 55 54 of plants) (days) seeding to once over harvest(days) 109 114 9. Adaptation culture field field 10. Chemistry andComposition of Full-Ripe Fruits pH 4.4 4.3 titratable acidity, as %citric 0.36 0.41 total solids (dry matter, seeds and skin 5.3 4.8removed) (percentage total content) soluble Solids as °Brix 4.6 4.2*These are typical values. Values may vary due to environment. Othervalues that are substantially equivalent are also within the scope ofthe invention.

C. Breeding Tomato Plants

One aspect of the current invention concerns methods for producing seedof tomato hybrid SVTM2303 involving crossing tomato lines PSQ9Z14-9091and PSQ9Z12-9063. Alternatively, in other embodiments of the invention,hybrid SVTM2303, line PSQ9Z14-9091, or line PSQ9Z12-9063 may be crossedwith itself or with any second plant. Such methods can be used forpropagation of hybrid SVTM2303 and/or the tomato lines PSQ9Z14-9091 andPSQ9Z12-9063, or can be used to produce plants that are derived fromhybrid SVTM2303 and/or the tomato lines PSQ9Z14-9091 and PSQ9Z12-9063.Plants derived from hybrid SVTM2303 and/or the tomato lines PSQ9Z14-9091and PSQ9Z12-9063 may be used, in certain embodiments, for thedevelopment of new tomato varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SVTM2303 followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSVTM2303 and/or tomato lines PSQ9Z14-9091 and PSQ9Z12-9063 for thepurpose of developing novel tomato lines, it will typically be preferredto choose those plants which either themselves exhibit one or moreselected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, high seed yield, high seedgermination, seedling vigor, high fruit yield, disease tolerance orresistance, adaptability for soil and climate conditions, and delayedfruit ripening. Consumer-driven traits, such as a fruit shape, color,texture, and taste are other examples of traits that may be incorporatedinto new lines of tomato plants developed by this invention.

Delayed fruit ripening is a trait that is especially desirable in tomatoproduction, in that it provides a number of important benefits includingan increase in fruit shelf life, the ability to transport fruit longerdistances, the reduction of spoiling of fruit during transport orstorage, and an increase in the flexibility of harvest time. The abilityto delay harvest is especially useful for the processing industry astomato fruits may be picked in a single harvest. A number of genes havebeen identified as playing a role in fruit ripening in tomato. Mutationsin some of these genes were observed to be correlated with an extendedshelf life phenotype (Gang et al., Adv. Hort. Sci. 22: 54-62, 2008). Forexample, WO2010042865 discloses that certain mutations in the 2nd or 3rdexon in the non-ripening (NOR) gene can result in extended shelf lifephenotypes. These mutations are believed to result in an early stopcodon. It is therefore expected that any mutation resulting in an earlystop codon in an exon in the NOR gene, particularly a mutation resultingin an early stop codon in the 3rd exon, will result in a slower ripeningor an extended shelf life phenotype in tomato. A marker to select forthe unique mutation of each allele and to distinguish between thedifferent alleles of the NOR gene in a breeding program can be developedby methods known in the art.

D. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

E. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theon. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

F. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS color chart byidentifying the group name, sheet number and letter, e.g., Yellow-OrangeGroup 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a tomatovariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation.

G. Deposit Information

A deposit of tomato hybrid SVTM2303 and inbred parent lines PSQ9Z14-9091and PSQ9Z12-9063, disclosed above and recited in the claims, has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The dates of deposit were Oct. 31,2016, Oct. 31, 2016, and May 5, 2015. The accession numbers for thosedeposited seeds of tomato hybrid SVTM2303 and inbred parent linesPSQ9Z14-9091 and PSQ9Z12-9063 are ATCC Accession No. PTA-123582,PTA-123579, and PTA-122151, respectively. Upon issuance of a patent, allrestrictions upon the deposits will be removed, and the deposits areintended to meet all of the requirements of 37 C.F.R. § 1.801-1.809. Thedeposits will be maintained in the depository for a period of 30 years,or 5 years after the last request, or for the effective life of thepatent, whichever is longer, and will be replaced if necessary duringthat period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A plant of tomato hybrid SVTM2303, a sample ofseed of said hybrid SVTM2303 having been deposited under ATCC AccessionNumber PTA-123582.
 2. A seed that produces the plant of claim
 1. 3. Aplant part of the plant of claim
 1. 4. The plant part of claim 3,wherein said plant part is a leaf, an ovule, pollen, a fruit, or a cell.5. A tissue culture of regenerable cells of the plant of claim
 1. 6. Thetissue culture according to claim 5, comprising cells or protoplastsfrom a plant part selected from the group consisting of embryos,meristems, cotyledons, pollen, leaves, anthers, roots, root tips,pistil, flower, seed and stalks, wherein said cells or protoplastscomprise a cell or protoplast of said hybrid SVTM2303.
 7. A tomato plantregenerated from the tissue culture of claim 5, wherein said plant hasall of the morphological and physiological characteristics of saidtomato hybrid SVTM2303.
 8. A method of vegetatively propagating thetomato plant of claim 1, the method comprising the steps of: (a)collecting tissue capable of being propagated from the plant accordingto claim 1; (b) cultivating said tissue to obtain proliferated shoots;and (c) rooting said proliferated shoots to obtain a rooted plantlet. 9.The method of claim 8, further comprising growing at least a firsttomato plant from said rooted plantlet.
 10. A method of producing atomato plant comprising an added trait, the method comprisingintroducing by genetic transformation a transgene conferring the traitinto the plant of claim
 1. 11. A tomato plant produced by the method ofclaim 10, wherein said plant comprises said transgene and otherwise allof the morphological and physiological characteristics of said hybridSVTM2303.
 12. A plant of tomato hybrid SVTM2303, a sample of seed ofsaid hybrid SVTM2303 having been deposited under ATCC Accession NumberPTA-123582, further comprising a transgene.
 13. The plant of claim 12,wherein the transgene confers a trait selected from the group consistingof male sterility, herbicide tolerance, insect resistance, pestresistance, disease resistance, modified fatty acid metabolism,environmental stress tolerance, modified carbohydrate metabolism andmodified protein metabolism.
 14. A plant of tomato hybrid SVTM2303, asample of seed of said hybrid SVTM2303 having been deposited under ATCCAccession Number PTA-123582, further comprising a single locusconversion.
 15. The plant of claim 14, wherein the single locusconversion confers a trait selected from the group consisting of malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism and modified proteinmetabolism.
 16. A method for producing a seed of a tomato plant derivedfrom tomato hybrid SVTM2303, the method comprising the steps of: (a)crossing the tomato plant of claim 1 with itself or a second tomatoplant; and (b) allowing a seed of a hybrid SVTM2303 derived tomato plantto form.
 17. A method of producing a seed of a hybrid SVTM2303 derivedtomato plant, the method comprising the steps of: (a) producing a hybridSVTM2303 derived tomato plant from a seed produced by crossing thetomato plant of claim 1 with itself or a second tomato plant; and (b)crossing the hybrid SVTM2303 derived tomato plant with itself or adifferent tomato plant to obtain a seed of a further hybrid SVTM2303derived tomato plant.
 18. The method of claim 17, further comprisingproducing a tomato plant grown from the seed of said step (b) andcrossing said tomato plant with itself or a different tomato plant toproduce a seed of a further hybrid SVTM2303-derived tomato plant.
 19. Amethod of producing a tomato fruit, the method comprising: (a) obtainingthe plant according to claim 1, wherein the plant has been cultivated tomaturity; and (b) collecting a tomato fruit from the plant.